Contents: 1. Introduction to animal cell culture. 2. Initiation of cell culture. 3. Growth of cells in culture. 4. Importance of aseptic techniques in cell culture. 5. Culture environment and culture media. 6. Maintenance and storage of culture. 7. Cell line banking. 8. Cell quantitation methods. 9. Cytotoxicity assays. 10. Scaling up of cultures. 11. General strategy for the production of monoclonal antibody. 12. Somatic cell nuclear transfer. 13. Genetic manipulation of animals. 14. Stem cells. 15. Glossary. References. Index.

Animal cell and tissue culture has been one of the foremost techniques paving the way for recent cutting edge technologies such as vaccinology, monoclonal antibody production, therapeutic cloning, stem cell technology, etc. This book covers a formal introduction and provides the basics in understanding the concept of tissue culture traversing through the state of the art technologies such as stem cell, hybridoma technology, transgenesis, cloning and scaling up technologies. This book will impart up to date knowledge and confidence to execute tissue culture related work with ease.