Contents: Preface. 1. Basic bioinformatics tools for molecular data analysis/K.N. Raja and I. Ganguly. 2. Data mining in animal genetics/Sachinandan De, Meenu Chopra and Biswajit Brahma. 3. Designing and In silico quality checking of PCR primers/C.S. Mukhopadhyay. 4. Sequence alignment: concepts and methods/C.S. Mukhopadhyay and Sarabjot Singh Osahan. 5. Submitting Nucleotide sequence to Bankit/C.S. Mukhopadhyay. 6. Molecular phylogeny: basics, methods and applications/C.S. Mukhopadhyay and Sarabjot Singh Osahan. 7. Real-time PCR for quantification of mRNA levels/Indrajit Ganguly, Anita Ganguly, Sanjeev Singh and Harishankar Singha. 8. Next-generation sequencing technologies: a novel approach for SNP genotyping studies/Soumendu Chakravarti. 9. Transcriptome analysis: methods and applications/Rafeeque R. Alyethodi, Rani Alex, Umesh Singh, Sushil Kumar and Rajib Deb. 10. Genome annotation in Prokaryotes and Eukaryotes/Sarika, Mir Asif Iquebal, C.S. Mukhopadhyay, Prakash G. Koringa, Anil Rai, Chaitanya G. Joshi and Dinesh Kumar. 11. DNA signature of agricultural germplasm: a computational approach/Mir Asif Iquebal, Sarika, Anil Rai and Dinesh Kumar. 12. Marker based technologies: a paradigm shift in selection methodologies in livestock/Rani Alex, Rafeeque R. Alyethodi, Umesh Singh, Sushil Kumar and Rajib Deb.
Rapid advancements in the molecular biology and genomics especially various genome sequencing projects, created a new major area of interest to the biologists. Bioinformatics, where biology merges with the computer science and information technology. Bioinformatics continue to be the main focus of attraction in the coming years, especially in the livestock production, as it will find more application in diagnostics, vaccine development, veterinary medicine, animal breeding and other disciplines of animal sciences. Also the advancements in next generation sequencing technologies will assist the animal scientists to understand the cellular and molecular mechanisms under different environmental conditions.
This book acquaints the readers with the basic bioinformatic tools which are essential for any molecular biology work like various databases, Primer designing sequence alignment, submission to Bankit and molecular Phylogeny analysis. Further the book also introduces and details various advanced topics like real time PCR, Next generation sequencing, transcriptome analysis genome annotation, computational approach for development of DNA signature and applications of marker based technologies in animal breeding. The Simplicity of language and clear illustration in various bioinformatics softwares will help even a beginner to learn the tools with a clear understanding. The concise and clearly written book will provide invaluable resources for both the beginners and established researchers in various fields of animal sciences, as well acquaintance with basic and advanced bioinformatics and in-silico tools can be acquired.
