Contents: UNIT-1 (Introduction, History and Applications of Microbiology). 1. Introduction, History of Microbiology (i) Discoveries Period (ii) Transition Period (iii) Golden Era, Fermentation, Medicine Theory (i) The Germ Theory of Disease (ii) Vaccination and Wonder Drug, Some Important Contributors in Microbiology (i) Antony Van Leeuwenhoek (ii) Louis Pasteur (iii) Robert Koch (iv) Sir Alexander Fleming (v) Selman Abraham Waksman (vi) Edward Jenner, Applications of Microbiology 2. Sterilization and Culture Medium 3. Isolation and Purification of Bacteria and Preservation of microorganism 4. Identification of an Unknown Microorganism 5. Stain and Staining Methods UNIT-2. (A General Account on Micro-organisms) 6. Algae 7: Fungi. 8. Bacteria 9. Virus 10: Mycoplasma. 11: Actinomycetes UNIT-3 (Genetic Recombination, Applications of Genetic Engineering, Selection of Mutants, Fermenter and Fermentation Process). 12. Genetic Recombination (Transfer of Genetic Material) (i) Transformation/ (ii) Transduction/ (iii) Conjugation. 13. Applications of Genetic Engineering. 14: Selection of Mutants. (i) Replica Plating Method. (ii) Gradient-Plate Method. (iii) Substrate Utilization Test. (iv) Ames Test. 15. Fermenters (Bioreactors), Fermentation Process and Role of Computer in Fermentation Technology. UNIT-4 (Instrumentation). 16. Microscope and Microscopy (i) Light Microscope: (a) Bright Field Microscope (b) Phase Contrast Microscope (c) Dark Field Microscope (d) Fluorescence Microscope (ii) Electron Microscope: (a) Transmission Electron Microscope (b) Scanning Electron Microscope. 17: Spectrophotometer and Colorimeter. 18. Chromatography: (i) Paper Chromatography (ii) Thin-Layer (Planar) Chromatography (iii) Gel Filtration Chromatography (iv) Ion Exchange Chromatography (v) Affinity Chromatography (vi) Adsorption Chromatography (vii) Partition Chromatography (viii) Gas-Liquid Chromatography (ix) Column Chromatography. 19. Electrophoresis, Radioactive Isotope Technique (i) Autoradiography (ii) Phosphoroimaging, Fluoremetry, Densitometery, Centrifuge. 20. pH Meter, Autoclave, Laminar Air Flow, Water Bath, Hot Air Oven, Incubator, Quebec Colony Counter, Micrometery, Balance UNIT-5 (Biostatistics). 21: Introduction: (i) Aims of Biostatistics (ii) Applications (iii) Use of Decimal Logarithm and Antilogarithm. 22. Central Tendency: Mean, Median and Mode. 23. Correlation and Regression. 24. Chi-Square Test and Analysis of Variance (ANOVA). 25. Probability. 26. Sampling Techniques.
Microbiology is the branch of biology that deals with the study of microscopic organism (microbes). Microbes are very important part of our life and are useful in our day-to-day activities. The microbiology is subdivided into various fields such as bacteriology, virology, protozoology, phycology and mycology. The advancement in the knowledge of the microbes and their practical utility in the field of medical microbiology, food microbiology, biofertilizers and plant growth promoters including bacteria and fungi, industrial microbiology and various other activities including various technological advancement and recent development in the food industry, agricultural productivity, pharmaceutical industry, environmental protection and abatement of pollution etc and genetic engineering in the field of microbes had helped in the development of the country. Since the research and advancement in teaching material is a continuous process and there is a need to include various research findings that had compelled the authors for inclusion of new advancement in various fields have been included in this book. It contains five different units including Unit 1-Introduction, history and applications of microbiology, Unit 2-General accounts of micro-organism, Unit 3. Genetic recombination, application of genetic engineering and fermentation, Unit 4-Studies of various instruments useful in microbiological laboratory and Unit 5. deals with biostatistics. This book will be useful to the undergraduate students in microbiology and can be used as a basic subject material for the students, teachers engaged in academics, research in different technical institutes and covers the course of different universities. There is always ample scope of improvement in the concerned subject material and various suggestion for improvement are welcomed by the authors and your suggestions will definitely help us to improve the standard of the book in the next editions and will act as a stimulus for further advancement.
