Contents: Preface. I. Basic techniques-cell and tissue culture of model species: 1. Media preparation/O.L. Gamborg. 2. The initiation and maintenance of callus cultures of carrot and tobacco/R.D. Hall. 3. The initiation and maintenance of plant cell suspension cultures/R.D. Hall. 4. Shoot cultures and root cultures of tobacco/J.F. Topping and K. Lindsey. 5. Somatic embryogenesis in orchardgrass/M.E. Horn. 6. Arabidopsis regeneration and transformation (leaf and cotyledon explant system)/R. Schmidt and L. Willmitzer. 7. Arabidopsis protoplast transformation and regeneration/B. Damm and L. Willmitzer. 8. Arabidopsis regeneration and transformation (root explant system)/D. Valvekens, M. Van Lijsebettens and M. Van Montagu. 9. Somatic embryogenesis in carrot/A.D. Krikorian and D.L. Smith. 10. Low density cultures: microdroplets and single-cell nurse cultures/G. Spangenberg. 11. Tobacco protoplast isolation, culture and regeneration/I. Negrutiu. II. Tissue culture and transformation of crop species: 1. Embryogenic callus, cell suspension and protoplast cultures of cereals/I.K. Vasil and V. Basil. 2. Transformation and regeneration of rice protoplasts/J. Kyozuka and K. Shimamoto. 3. Transformation and regeneration of orchardgrass protoplasts/M.E. Horn. 4. Transformation and regeneration of oilseed rape proplasts/D. Rouan and R. Guerche. 5. Regeneration and transformation of potato by Agrobacterium tumefaciens/R.G.F. Visser. 6. Transformation of tomato with agrobacterium tumefaciens/S. McCormick. 7. Regeneration and transformation of sugarbeet by agrobacterium tumefaciens/K. Lindsey, P. Gallios and C. Eady. 8. Regeneration and transformation of apple (Malus Pumila Mill.)/D.J. James and A.M. Dadekar. 9. Transformation and regeneration of maize protoplasts/C.A. Rhodes and D.W. Gray. 10. Regeneration and transformation of barley protoplasts/P. Lazzeri. 11. Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation/J.P. Spychalla and M.W. Bevan. 12. Production of fertile transgenic wheat by microprojectile bombardment/Dirk Becker and Horst Lorz. 13. Transient gene expression and stable genetic transformation into conifer tissues by microprojectile bombardment/Armand Seguin. III. Propagation and conservation of germplasm: 1. Clonal propagation of orchids/Y. Sagawa. 2. Clonal propagation of palms/B. Tisserat. 3. Clonal propagation of conifers/T.A. Thorpe and I.S. Harry. 4. Cytological techniques/A. Karp. 5. Restriction fragment analysis of somaclones/R.H. Potter. 6. Virus elimination and testing/M.C. Coleman and W. Powell. 7. Clonal propagation of citrus/T.S. Rangan. 8. Clonal propagation of eucalypts/J.A. McComb. 9. Cryopreservation of plant tissue cultures: the example of embryogenic issue cultures from conifers/P.J. Charest, J. Bongs and K. Klimaszewska. IV. Direct gene transfer and protoplast fusion: 1. Gene transfer by particle bombardment/T.M. Klein, S. Knowlton and R. Arentzen. 2. Transformation of pollen by particle bombardment/D. Twell, T.M. Klein and S. McCormick. 3. Electrical fusion of protoplasts/M.G.K. Jones. 4. Cybrid production and selection/E. Galun and D. Aviv. 5. Fluorescence-activated analysis and sorting of protoplasts and somatic hybrids/D.W. Galbraith. 6. RFLP analysis of organellar genomes in somatic hybrids/E. Pehu. 7. Isolation and uptake of plant nuclei/P.K. Saxena and J. King. 8. In situ hybridization to plant metaphase chromosomes: radioactive and non-radioactive detection of repetitive and low copy number genes/J. Veuskens, S. Hinnisdaels and A. Mouras. 9. Chemical fusion of protoplasts/P. Anthony, R. Merchant, N.W. Blackhall, J.B. Power and M.R. Davey. V. Reproductive tissues: 1. In vitro fertilisation of maize/E. Kranz. 2. Endosperm culture/S. Stirn and H.J. Jacobsen. 3. Endosperm culture/B.M. Johri and P.S. Srivastava. 4. Hybrid embryo rescue/A. Agnihotri. 5. In vitro culture of Brassica Juncea zygotic proembryos/Chun-ming Liu, Zhi-hong Xu and Nam-Hai Chua. 6. Production of haploids in Brassica spp. via microspore culture/A.M.R. Ferrie and W.A. Keller. VI. Mutant selection: 1. Use of chemical and physical mutagens in vitro/P.J. Dix. 2. In vitro culture, mutant selection, genetic analysis and transformation of physcomitrella patens/David Cove. VII. Secondary metabolites: 1. Tropane alkaloid biosynthesis in vitro/R. Robins. 2. Anthocyanin biosynthesis in vitro/A. Komamine and K. Kakegawa. 3. Biosynthesis of monoterpene indole alkaloids in vitro/W.G.W. Kurz. K. Constabel and R. Tyler. VIII. Tissue culture techniques for fundamental studies: 1. Establishment of photoautotrophic cell cultures/W. Husemann. 2. Zinnia mesophyII culture system to study xylogenesis/M. Sugiyama and H. Fukuda. 3. Cell cycle studies: induction of synchrony in suspension cultures of catharanthus roseus cells/H. Kodama and A. Komamine. 4. Thin Cell Layer (TCL) method to programme morphogenetic patterns/K. Tran Thanh Van and C. Gendy. 5. In vitro infection of arabidopsis with nematodes/Joke C. Klap and Peter C. Sijmons. 6. Asparagus cell cultures as a source of wound-inducible genes/R.M. Darby and J. Draper. 7. Use of video cell tracking to identify embryogenic cultured cells/Marcel A.J. Toonen and Sacco C. de Vries. Index.
From the Preface: "Plant tissue culture has a long history, dating back to the work of Gottlieb Heberlandt and others at the end of the 19 century, but the associated concepts and techniques have reached a level of usefulness and application which has never been greater. The technical innovations have given new insights into fundamental aspects of plant differentiation and development, and have paved the way to the identification of strategies for the genetic manipulation of plants. It is the aim of this manual to deliver a broad range of these techniques in a form which is accessible to students and research scientists of diverse backgrounds, including those with little or no previous experience. The themes of the manual aim to reflect those research areas which have been advanced by tissue culture technology.
The objective has been from the start to produce a manual which is at home on the laboratory bench. The plastic-covered, ring-bound format has proved to be most popular and is retained here. Equally, the emphasis has been on producing a collection of detailed step-by-step protocols, each supplemented with an introductory text and practical footnotes, to provide the next best thing to a supervisor at one's shoulder. Each author was chosen as one actively using the respective technique in his or her own laboratory, in order to give an authoritative account, with the most common difficulties or pitfalls highlighted for the benefit of the newcomer to the field."