Contents: 1. Introduction to DNA sequencing: Sanger and beyond/Brian K. Nunnally. 2. Developments in the detection of DNA sequencing fragments using fluorescence: Energy transfer and fluorescence lifetimes/Brian K. Nunnally. 3. Microscale sample preparation for DNA sequencing and genotyping/Yonghua Zhang and Edward S. Yeung. 4. Microfabricated DNA sequencing devices/Brian M. Paegle, Robert G. Blazej and Richard A. Mathies. 5. Analysis of nucleic acids by mass spectrometry/Dirk Van Den Boom and Franz Hillenkamp. 6. Sequencing the single DNA molecule/Kenneth D. Weston and Markus Sauer. 7. DNA sequencing for genome analysis/Jeffrey P. Tomkins, Todd C. Wood and Dorrie Main. 8. Sequence reconstruction from nucleic acid microarray data/Franco P. Preparata, Eli Upfal and Samuel A. Heath. 9. Ancient DNA/William Goodwin. 10. Forensic DNA sequencing/Terry Melton and Victor W. Weedn. Index.

"While one marvels at the DNA sequences derived from the Human Genome Project, perhaps just as significant and fascinating is the development of the amazing analytical technology that has permitted us entry into the genomic era and a whole new level of scientific understanding.

Analytical Techniques in DNA Sequencing takes a look at the various DNA sequencing techniques that were developed and put to use during and after the Human Genome Project. The book pays special attention to the breakthrough Sanger Method, as well as several other techniques that improved the accuracy, improved the detection limits, and in a variety of ways, dramatically reduced the time needed to generate a DNA sequence. This volume also delves into the far-reaching applications of DNA sequencing including:

• DNA typing for forensics.
  
• Sequencing ancient DNA.
  
• Genome analysis.
  
• Sequencing the single molecule.

The book's ten chapters include contributions by a variety of leading experts in the field. They present all of the major improvements made since the early days of radioisotope sequencing.

A few features found in Analytical Techniques in DNA Sequencing

• Considers major developments in the detection of sequencing fragments.
  
• Covers a variety of DNA sequencing methods including a complete discussion of Sanger and Maxam Gilbert based sequencing.
  
• Explains a wide variety of disciplines including fluorescence spectroscopy, mass spectrometry and capillary electrophoresis.
  
• Looks at chip-based microfabricated sequencing systems, the future of DNA sequencing."