Antibodies : A Laboratory Manual

Contents: Preface. 1. Immune response. 2. Antibody molecules. 3. Antibody--antigen interactions. 4. Antibody response. 5. Immunizations. 6. Monoclonal antibodies. 7. Growing hybridomas. 8. Storing and purifying antibodies. 9. Labeling antibodies. 10. Cell staining. 11. Immuno-precipitation. 12. Immunoblotting. 13. Immuno affinity purification. 14. Immunoassay. 15. Reagents. Appendices. References. Index.

From the preface: "This manual was inspired by "the cloning manual," Molecular Cloning: A Laboratory Manual by Tom Maniatis, Ed Fritsch, and Joe Sambrook, a book that did so much to expand the community of scientists using molecular biology methods. The great joy of that manual was and is the confidence it gives to beginners and the way it tempts all workers to try new to approaches. At present, many of the methods of modern immunochemistry seem beyond the reach of nonimmunologists, and one of the goals of Antibodies is to make these methods accessible to a wider group. This manual is judged by how well it succeeds in breaking this barrier."