Biophysical Chemistry : Metal Ions DNA Interaction

Contents: 1. Introduction. 2. DNA structure. 3. Optical properties of DNA. 4. Denaturation. 5. Renaturation. 6. Holix coil transition in DNA. 7. Importance of metal ions. 8. Interaction of DNA with metal ions. 9. Metals interacting at. 10. Phosphate group A. 11. Consequences of metal binding. 12. To phosphates. 13. Metal interacting with bases. 14. Consequences of metal ions. 15. Binding at bases. 16. Metal interacting at the phosphate and bases. 17. Consequences of the metal binding to the bases and phosphates. 18. The biochemical and biological significance of DNA. 19. Metal cation interaction. 20. Aim of the present investigation. 21. Electrophoresis and sedimentation. 22. Theory of electrophoresis. 23. Theory of sedimentation potential. 24. Dielectric studies. 25. Maxwell-Wagner theory. 26. Counter ion polarisation. 27. Solvent effect. 28. Volume change on mixing. 29. Materials, methods and results. 30. Materials. 31. Method: i. Electrophoresis. ii. Preparation of AG-AG-AGCI electrode. 32. Power supply unit. 33. Preparation of particles. 34. Preparation of medium. 35. Electrophoretic mobility. 36. Sedimentation potential. 37. Calibration of amplifier meter. 38. Dielectrics. 39. Volume change on mixing. 40. Spectrophotometric studies. 41. Results. 42. Discussion. 43. Discussion of the model. Summary. References.