Biophysical Chemistry: Techniques and Applications
Contents: Part-I: Different Types of Biomolecules. 1. Cell and its biochemical setup. 2. Thermodynamic aspects of the cell. 3. Carbohydrates, their reactions, thermochemistry and energetics. 4. Lipids. 5. Amino acids. 6. Peptides. 7. Proteins. 8. Nucleosides and nucleotides. 9. Enzymes. 10. Co-enzymes and Vitamins. 11. Bioenergetics. 12. Biosensors. Part-II: Bioanalytical techniques. 13. Surface Plasmon resonance spectroscopy. 14. Affinity chromatography. 15. Capillary Electrophoresis. 16. NMR technique in the elucidation of biochemical problems. 17. Applications of ESR technique for the solution of biological problems. 18. Flow methods for the kinetic study of fast biochemical systems. 19. Temperature jump relaxation technique for the kinetic study of fast biochemical reactions. 20. Flash photolysis technique for the kinetic study of fast biochemical reactions. 21. Pressure Jump relaxation method for the study of fast biochemical reactions. 22. Circular dichroism as a tool for the analysis of biochemical reactions. 23. Applications of isothermal calorimetry in the study of biochemical reactions. 24. Principles of differential scanning calorimetry and its applications to the study of biochemical reactions. 25. Applications of gel filtration technique in the separation of biomolecules. 26. Principles of gel electrophoresis and its applications to biochemical analysis. 27. Analytical ultracentrifugation method in the analysis of biomolecules. 28. Ion exchange chromatography and its applications in the separation of biomolecules. 29. Principles of surface enhanced Raman scattering and its bio analytical applications. 30. Mass spectroscopy and its applications in the analysis of biomolecules. 31. X-ray studies in the elucidation of the structure of bio molecules. 32. CRISPR –CAS9- A method for genome editing.
The book ‘Biophysical Chemistry’ provides a pedagogical account of diverse topics of biophysical chemistry ranging from the structure of cells, lipids and proteins to nonequilibrium thermodynamics, enzyme catalysis and fast reaction kinetics. For convenience, the book is divided into two parts viz (i) Biomolecules and (ii) Biochemical Techniques. In order to cater to the needs of diverse spectrum of readers, the emphasis has been on the fundamental structural concepts in the first part (Chapters 1 to 12) while the second part (Chapters 13 to 32)provides a systematic account of different experimental techniques such as chromatography, electrophoresis, gel filtration etc that are extensively employed in biochemical research. A few typical applications using NMR, ESR and SPR are provided so as to comprehend the power of spectroscopic tools in biophysical chemistry. Starting from an elementary treatment of structural organic chemistry, the book deals with advanced topics of entropy production and biosensors.
The book consists of a large number of schematic diagrams as well as tabular compilations of kinetic, electrochemical and thermochemical data. In view of the diverse coverage of topics, this book will be a valuable asset to undergraduate and post graduate students of chemistry, biology and medicine. An unique feature of the book is the inclusion of the multiple choice questions, numerical examples and descriptive questions in various chapters.
