Crop Physiology

Contents: 1. Preparation of solutions. 2. To demonstrate the phenomenon of osmosis using potato osmoscope. 3. To demonstrate the phenomenon of endosmosis and exosmosis by using grapes and raisins. 4. To demonstrate the process of diffusion. 5. Methods of measurement of water status and water saturation deficit in plant parts. 6. Estimation of water potential of plant tissue by Chardakov’s method. 7. Estimation of photosynthetic pigment from plant leaves. 8. Measurement of absorption spectrum of chloroplastic pigments. 9. To demonstrate chlorophyll fluorescence. 10. Measurement of leaf area by graph paper method. 11. Estimation of stomatal index and stomatal frequency with replica method. 12. Demonstration of leaf anatomy or structure of C3 and C4 plants (demonstration by already prepared slides). 13. To demonstrate that water is lost from a living plant during transpiration. 14. To measure rate of transpiration of potted plant by gravimetric (lysimeter) method. 15. To measure the rate of transpiration by using Ganong’s photometer. 16. To find out the relation between transpiration and absorption. 17. To demonstrate unequal transpiration by the two leaf surfaces from four leaf method. 18. To demonstrate that light and carbondioxide are essential for photosynthesis (moll’s half leaf experiment). 19. To demonstrate that O2 is evolved during photosynthesis. 20. To demonstrate anaerobic respiration. 21. To demonstrate that oxygen is used up during respiration. 22. To determine respiratory quotient of different types of respiratory substrates. 23. To demonstrate the optimum conditions for seed germination. 24. Breaking of seed dormancy to facilitate germination. 25. Determination of the seeds viability by tetrazolium test. 26. To demonstrate the measurement of growth in plants with the help of arc auxanometer. 27. Growth analysis: calculation of growth parameters. 28. Yield estimation of Kharif crops. 29. Effect of ethylene on regulation of stomata.

The objective of this book is to bring into focus the practical application of the theoretical knowledge of crop physiology. This book on Crop Physiology describes basic principles and procedures with exercises dealing with demonstration of important physiological and metabolic processes viz. osmosis, diffusion, water potential, stomatal frequency and index, plant water status, water relation, Measurement of absorption spectrum of chloroplastic pigments and fluorescence, leaf area, plant growth and transpiration. Demonstration of photosynthesis, transpiration, relationship between absorption and transpiration, conditions for seed germination, seed viability and dormancy aspects, chemical solution preparation for physiological and biochemical analysis of plant samples.