DNA Science: A First Course
Contents: Preface. Acknowledgments. 1. How we Learned that DNA is the Genetic Material. 2. How we Learned the Function of DNA. 3. How we Learned how Genes are Regulated. 4. Basic Tools and Techniques of DNA Science. 5. Methods for finding and Expressing Important Genes. 6. Modern Methods for Analyzing whole Genomes. 7. The DNA Science of Cancer. 8. Applying DNA Science to Human Genetics and Evolution. 9. LABORATORIES: Lab Safety and Adherance to National Institute of Health Guidelines: Measurements, Micropipetting, and Sterile Techniques. 10. Bacterial Culture Techniques: A. Isolation of Individual Colonies B. Overnight Suspension Culture C. Mid-log Suspension Culture. 11. DNA Restriction Analysis. 12. Effects of DNA Methylation on Restriction. 13. Rapid Colony Transformation of E. Coli with Plasmid DNA. 14. Assay for an Antibiotic Resistance Enzyme. 15. Purification and Identification of Recombinant GFP: A. Plasmid Minipreparation of pAMP B. Restriction Analysis of Purified pAMP. 16. Recombination of Antibiotic Resistance Genes: A. Restriction Digest of Plasmids pAMP and pKAN. B. Ligation of pAMP and pKAN Restriction Fragments. 17. Transformation of E. Coli with Recombinant DNA: A. Classic Procedure for Preparing Competent Cells B. Transformation of E.coli with Recombinant DNA. 18. Replica Plating to Identify Mixed E.Coli Populations. 19. Purification and Identification of Recombinant DNA: A. Plasmid Minpreparation of pAMP/pKAN Recombinants. B. Restriction Analysis of Purified Recombinant DNA: Answers to Discussion Questions. APPENDICES: Equipment, Supplies, and Reagents. Recipes for Media, Reagents, and Stock Solutions. Restriction Map Data for pAMP, pKAN, pBLU, pGREEN, and Bacteriophage l. Cautions. Name Index. Subject Index.
"This is the second edition of a very successful textbook that combines an illustrated, narrative text with 12 rigorously tested, reliable lab experiments in molecular biology, developed at the internationally renowned Dolan DNA Learning Center of Cold Spring Harbor Laboratory. Proven through more than 10 years of teaching in research and non-research colleges and universities, junior colleges, community colleges, and advanced high school biology programs, DNA Science has been successfully integrated into introductory biology, general biology, genetics, microbiology, cell biology, molecular genetics and molecular biology courses. The Text has been completely revised, extensively rewritten, and updated. The new coverage extends to the completion of the draft sequence of the human genome and the enormous impact these and other sequence data are having on medicine, research, and industry. The laboratory experiments cover basic techniques of gene isolation and analysis that will be successful even in the hands of teachers and students with no prior experience. Extensive prelab notes at the beginning of each experiment explain how to schedule and prepare, while flowcharts and icons make the protocols easy to follow. The laboratory course is completely supported by quality-assured products from the Carolina Biological Supply Company, from bulk reagents, to usable reagent systems, to single-use kits, thus satisfying a broad range of teaching applications."
