Electron Microscopy

Contents: Instrumentation: 1. Electron microscope-an introduction. 2. Electron microscope-principles, theories and comparison with optical microscope. 3. Parts of the transmission electron microscope. 4. Operation and maintenance of TEM. 5. Scanning electron microscopy. II. Specimen preparation techniques: I. Processing of tissue specimens: 6. Fixation. 7. Washing and dehydration. 8. Infiltration and embedding. 9. Sectioning (Semithin and ultrathin sections). 10. Staining. II. Processing of other types of specimens: 11. Paraffin block sections. 12. Cell cultures and fine needle aspirates. III. Other processing techniques: 13. Rapid processing, shadow casting and replica techniques. 14. Negative staining. 15. Microwave techniques in electron microscopy. 16. Cryotechniques in electron microscopy. III. Applications of TEM: 18. Artifacts in EM. 19. EM in biological research. 20. Electron microscopy in tumour diagnosis. 21. EM in diagnosis of diseases other than tumours. 22. EM microbiology. 23. EM in oral pathology. 24. Applications in nanoscience. IV. Recent advances: 25. An introduction to modern microscopes. Appendix. Glossary. Index.

The advent of electron microscopes has opened up new vistas in the field of science. The ultra-structural morphological evidence offered by electron microscope to substantiate and support other findings is highly rewarding. This book gives a comprehensive overview of the principle and operations of the electron microscope. Numerous electron micrographs have been provided to acquaint the reader with the appearance of highly magnified features seen through the EM. This book would definitely create a feel for this subject particularly among those who want to use this technique for their research work.