Experimental Plant Biotechnology Techniques

Contents: Foreword. Preface. Introduction. Part. I. Laboratory setup and role of growth regulators and nutrients in plant tissue culture media: 1. Plant tissue culture laboratory organization : the technological advancements. 2. Role of plant hormones in tissue culture. 3. Role of nutrients in plant tissue culture. 4. Handling and standardization of plant material. Part. II. Preparation of culture media, sub-culturing of cell and suspension culture in test tube breeding and protoplast technology for genetic manipulation: 5. Preparation of tissue culture media. 6. Isolation, inoculation and sub-culturing of callus for cell and suspension cultures. 7. Estimation of growth kinetics of cultured cells. 8. Test tube breeding for mass clonal propagation.  9. Protoplast technology: isolation and culture. III. Genetic manipulation techniques: using in vitro protoplast fusion and gene transfer methods for transformation: 10. Gene transfer methods. 11. Characterization of somatic hybrids and transformation technologies. Appendices. Suggested readings.

"The book "Experimental Plant Biotechnology Techniques" contains the ICAR syllabus for the postgraduate programmes which emphasize the lab activities in different areas of plant biotechnology, molecular biology and genetic engineering etc. This book covers all important aspects of practical utility in field of genetic manipulation by tissue culture methods, protoplast technology and gene transfer methods for providing the students simple and reproducible protocols so that the practical utility in different areas of biotechnology should be fulfilled. The present book is prepared for lab based activities and theoretical aspects of plant genetic manipulation techniques to be used in boosting crop production. Valuable information included in appendices." (jacket)