Histochemistry in Focus : A Sourcebook of Techniques and Research Needs

Contents: 1. Fixatives and methods of fixation. 2. Tissue processing. 3. Theory of staining. 4. Decalcification. 5. Preparation of stains. 6. Mountants. 7. Haematoxylin staining. 8. Frozen methods. 9. Carbohydrates. 10. Protein. 11. Amyloids. 12. Nucleic acids. 13. Lipids. 14. Pigments. 15. Minerals. 16. Microorganisms in sections. 17. Microorganisms in smears. 18. Enzymes. 19. Connective tissue. 20. Neurological studies. 21. Endocrine glands. 22. Haematological studies. 23. Preparation of permanent whole mounts of invertebrates or their parts. 24. Microwave histology. 25. Ultrahistochemistry. 26. Techniques in cell biology. 27. Methods for special organs. 28. Invertebrate staining methods. 29. Mast cells. 30. Immunocytochemistry. References. Subject index.

"Histology and histochemistry form the backbone of biochemistry and many aspects of life science subjects. This book presents a broad array of histochemical techniques applicable to the study of many cell and tissue types as well as to the constituents that are present both in invertebrate and vertebrate organisms. It would serve to be a simplified compendium which would not only enable students and researchers to learn the histochemical techniques and the theory behind each of them but would also serve as a sort of vade mecum. Many techniques have been supported with results from the authors studies in the form of colour photographs.

The techniques described would find use not only in research laboratories where basic questions are being investigated but also in medical and pathological laboratories where disease stages are being studied; these techniques will help to provide a better understanding of the changes that bring about pathological conditions." (jacket)