Laboratory Manual of Biochemistry

Contents: Preface. 1. Estimation of Titratable Acidity. 2. Estimation of Ascorbic Acid (Vitamin C). 3. Determination of Chlorophyll. 4. Determination of Carotene (Vitamin A). 5. Separation and Determination of Carotenoids. 6. Estimation of Total Soluble Sugar. 7. Estimation of Reducing Sugar. 8. Determination of Total Phenols. 9. Estimation of True Protein by Folin – Lowry’s Method. 10. Determination of Methionine. 11. Estimation of Proline. 12. Determination of Tryptophan. 13. Estimation of Pectin Substances. 14. Determination of Furfural. 15. Determination of Stevens Clouds Stability Test. 16. Determination of Ethanol. 17. Determination of Acetaldehyde (CH3CHO). 18. Carotenoids Estimation by HPLC. 19. Sugar Analysis by HPLC. 20. Organic Acid Determination by HPLC. 21. Determination of Thiabendazole in Fruit by UV  Spectrophotometer . 22. Determination of Peroxidase Enzyme (Donor : H2O2 Oxidoreductase EC 1.11.17). 23. Determination of Polyphenol Oxidase Enzyme (PPO) (Monophenol, Dihydroxy Phenylalanine Oxygen Oxidoreducaste EC 1.14.18.1). 24. Determination of Catalase Enzyme (Hydrogen Peroxide : Hydrogen Peroxide Oxidoreductase EC 1.11.1.6). 25. Isolation of Plant Genomic DNA by C-Tab Method. 26. SDS-PAGE Electrophoresis of Protein. Annexures. Index.

The book ‘Laboratory Manual of Biochemistry’ primarily designed for undergraduate and postgraduate Students of Biochemistry, Horticulture and Biotechnology,the book also be useful to professionals, researchers and entrepreneurs. This practical loboratory manual has been designed to familiarise students with such protocols with flow chart that can be understood easily.

Key Features:

Written in easy to understand style.
Provides simple clear and authoritative guide to the principles and scope of Biochemistry.