Methods in Plant Tissue Culture

Contents: 1. Plant Tissue Culture an Overview. 2. History of Plant Tissue and Cell Culture. 3. General Methodology of Plant Tissue Culture. 4. Culture Media Ingredients, Preparation and Related Problems. 5. Methods of Sterilization and Disinfestation. 6. Aseptic Techniques and Preparation of Explants. 7. Plant Tissue Culture Some Related Aspects. 8. Histological and Photographic Techniques for Plant Tissue Culture. 9. Protoplast Technology. 10. Micropropagation in Plants. 11. Micropropagation: Commercial Laboratory Production. 12. Somatic Embryogenesis Principles, Concepts and Applications. 13. Somaclonal and Gametoclonal Variant Selection. 14. Gynogenesis. 15. Synseeds or Synthetic Seeds. 16. Plant Tissue Culture and Germplasm Conservation. 17. Plant Tissue Culture and Secondary Metabolite Production. 18. Indexing for Plant Pathogens. 19. Eighteen Techniques for Crop Improvement. Abbreviations Acronyms, Glossary, Appendix and References.

Tissue culture is the ever-ready tool for specialists who hybridize plants by either sexual or asexual means. It is a clean and rapid way for genetic engineers to grow material for identifying and manipulating genes or to transfer individual characteristics from one plant to another. It plays a role in a wide array of fields, such as botany, chemistry, physics, genetic engineering, molecular biology, hybrid development, pesticide testing and food science. This book is being brought out to fill the gap in the available literature on plant tissue culture, especially focusing on practical procedures and protocols of tissue culture. (Jacket)