Methods of Bacterial Plant Pathology
Contents: Preface. Preamble: bacteria and their properties. 1. Sterilization of laboratory equipments and culture media. 2. Examination of living bacteria and isolation of pathogenic bacteria from host and soil. 3. Microscopic studies of infected host tissue. 4. Growth and measurement of growth of bacteria. 5. Staining methods of bacteria. 6. Techniques of inoculation with phytopathogenic bacteria. 7. Culture media. 8. Differential and selective media. 9. Biochemical characters. 10. Changes in chlorophyll a, b, carotenes and xanthophylls in infected plants. 11. Production of pectinolytic enzymes by plant pathogenic bacteria. 12. Preservation of diseased specimens. 13. Isolation of bacteriophage. 14. Preservation of bacterial culture. 15. Electron microscopic studies of bacteria. 16. Rhizobium cultures for seed treatment of legume crops : multiplication and method for testing of seeds. 17. Taxonomy of plant pathogenic bacteria and modern trends. 18. Important references on taxonomy of bacteria. 19. Measurement of PH. 20. Development of plant bacteriology in India. Appendix. Subject index.
"The book is intended as a reference book for practical classes of under graduate and post graduate students for studying bacterial diseases of plants. The book covers basic and applied aspects of the subject. The book begins with a brief description about bacteria and their properties of the twenty chapters, first five describe methods of sterilization of laboratory equipments and cultural media, isolation of pathogenic bacteria from host, microscopic studies of infected host, growth and measurement of bacteria and staining methods for bacteria. Next five chapters describes inoculation techniques, culture media, differential and selective media, biochemical characters, changes in chlorophyll a,b, carotenes and xanthophylls in infected plants. Next four chapters deal with production of pectinolytic enzymes by bacteria, preservatives for diseased specimen, isolation of bacteriophage and preservations of bacterial cultures. The next five chapters describes electron microscopic studies of bacteria Rhizobium culture for seed treatment, taxonomy of plant pathogenic bacteria and modern trends, some important references on taxonomy of bacteria and measurement of pH. The twentieth chapter deals with development of plant bacteriology in India." (jacket)
