Modern Analytical Techniques Used in Animal Nutrition Research

Contents:  Preface. Foreword. 1. Preparation and preservation of various types of solutions. 2. Design of experiments in animal nutrition research. 3. Determination of digestibility of feed stuffs. 4. Digestion and metabolism trials on experimental animals. 5. Estimation of proximate principles in feed samples. 6. Fibre fractionations and techniques of fibre analysis. 7. Estimation of soluble and structural carbohydrates. 8. Estimation of gross energy in biological samples. 9. Application of near infrared reflectance spectroscopy in feed analysis. 10. Estimation of methane emission in ruminants. 11. Estimation of Macro and micro minerals. 12. Atomic absorption spectrophotometry. 13. Estimation of anti-nutritional/toxic factors. 14. True protein and non-protein nitrogen fractions in biological materials. 15. Calorimetric determination of purine derivatives. 16. Estimation of Purine derivatives by high performance liquid chromatography. 17. Analysis of Rumen liquor samples. 18. Counting of Rumen protozoa by microscopic techniques. 19. Quantification of probiotic cultures. 20. Extraction of DNA and RNA gel electrophoresis and PCR of genomic DNA from Rumen liquor. 21. Estimation of bio-chemicals in blood and milk samples. 22. Determination of body composition of animals. 23. Conduction of digestion and metabolism trials. 24. Preparation of urea molasses mineral block and compressed complete feed blocks. Appendices.

This text represents an attempt to summarize and consolidate a considerable amount of information available in the literature with respect to laboratory analytical techniques which are commonly used in animal nutrition studies. There are few laboratory manuals on analytical techniques in Animal Nutrition written by renowned teachers and scientists of National Agriculture Research System of the country. However there is no single book of laboratory analytical techniques on animal  nutrition which fulfills the requirements of post graduate students of animals sciences by providing all the required latest information about various analytical methods at one place. The chapters are given in details with a candid and simple manner. Each practical method has been introduced with the principle and text materials which will orient the students with the subject. (jacket)