Molecular Biology and Biotechnology: Basic Experimental Protocols

Contents: Preface. I. Genomics: 1. Initial characterization of DNA. 2. DNA probe preparation southern blotting and hybridization. 3. RFLP and SSCP analysis of DNA. 4. Protein DNA interaction. 5. Plasmid DNA analysis. 6. Polymerase chain reaction. 7. RNA analysis techniques. 8. Gene or mRNA expression in cells/tissues. 9. Transcription activity in vivo. 10. Apoptosis analysis. II. Proteomics: 11. Initial preparations for protein analysis. 12. Quantitative determination of proteins. 13. Protein separation and analysis. 14. Electroblotting dot/slot blotting and electroelution. 15. Polyclonal antibodies production and immunoanalysis. 16. Protein-protein interactions. III. Quantitative biochemistry: 17. Analysis of biomolecules. 18. Enzyme kinetics and enzyme activity analysis. 19. Labelling of proteins and its uses. 20. Statistical analysis of biochemical data. IV. Bioinformatics: 21. Sequence retrieval from databases. 22. Sequence similarity search. 23. Protein sequence and structure analysis. 24. Nucleotide sequence analysis. 25. Molecular phylogeny. Annexes. References. Index.
 
Molecular biology and biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book genomics, proteomics, quantitative biochemistry, and bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.

Key Features:

Ready design of basic experiments in biomedical and biotechnology sciences.
Extensive coverage of basic equipment used in biomedical and biotechnological studies.
Concise information on each subject with focus on practical lessons.