Plant Biochemical and Physiological Methods

Contents: 1. Spectrophotometer. 2. Calibrating Spectrophotometer. 3. Sample Collection and Precautions for Optimal Sampling. 4. Estimation of Chloroplastic Pigment Contents. 5. Hill Activity. 6. Estimation of Carbohydrate Contents. 7. Sugar Metabolizing Enzymes. 8. Estimation of Oxidative Stress Markers. 9. Antioxidant Enzymes. 10. Assay of Glutathione Metabolizing Enzymes (Ascorbate-Glutathione Cycle). 11. Amino Acid Content. 12. Carbonyl Content Estimation. 13. Assay of Krebs Cycle Intermediates. 14. Extraction and Assay of Krebs Cycle Enzymes. 15. Extraction and Assay of Phosphorolytic Enzymes. 16. Extraction and Assay of GABA Synthesizing Enzymes and Estimation of GABA Contents. 17. Nitrate Reductase (NR) Activity. 18. Relative Water Content (RWC). 19. Membrane Stability Index (Sairam, 1994). 20. Protein Content (Lowry et al., 1951). 21. α-Amylase Activity. 22. Amylose Content. 23. Amylopectin. 24. Chlorophyll stability index (CSI). 25. Gas Exchange Parameters. 26. Biomass Accumulation and Allocation Pattern. 27. Germination Parameters. 28. Quantitative Drought Tolerant Parameters.