Proteins and Proteomics: A Laboratory Manual

Contents: Preface. Acknowledgements. Foreword. 1. Introduction to Proteomics. 2. One-dimensional Polyacrylamide Gel Electrophoresis. 3. Preparation of Cellular and Subcellular Extracts. 4. Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients. 5. Reversed-phase High-performance Liquid Chromatography. 6. Amino-and Carboxy-terminal Sequence Analysis. 7. Peptide Mapping and Sequence Analysis of Gel-resolved Proteins. 8. The Use of Mass Spectrometry in Proteomics. 9. Proteomic Methods for Phosphorylation Site Mapping. 10. Characterization of Protein Complexes. 11. Making Sense of Proteomics: Using Bioinformatics to Discover a Protein’s Structure, Functions, and Interactions. 12. Appendices. Reference Tables. Techniques. Cautions. Suppliers. Index.

"The Sequencing of genomes has opened the door for exploration of the proteome, the many thousands of proteins and their interactions in a given organism. Reliable, bench-tested protocols are required for identifying, isolating, and quantifying proteins in a given cell or pathway and for performing functional assays. Proteins and Proteomics: A Laboratory Manual by Richard Simpson, a leading authority in the field, provides the first authoritative, wide-ranging, technical approach to proteomics. Presenting a logical strategy for analyzing proteomes, Proteins and Proteomics provide background information about protein structure, as well as methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive supporting information and references are given on the theory of the techniques, as well as their applications. This manual is an essential laboratory tool for geneticists and molecular biologists studying phenotype and cellular function, and for protein chemists and biochemists seeking current techniques in this emerging field."