Seed Health
Contents: Part A - General: 1. Introduction: i. Terminology. ii. Historical development. iii. Seed industry and seed health in India. iv. Objectives of seed health testing. v. Ideal method. vi. Sampling and sample size in seed health. Part B - Techniques: 2. Examination of dry seeds: i. Visual observation of seeds. ii. Observations using a bright field microscope. iii. Observation under near-ultraviolet/UV light. iv. Use of adhesive tape. 3. Seed soak and washing test: i. Seed soak test. ii. Washing test. iii. Spore identification. 4. Culture methods: i. Incubation methods. ii. Dilution plate method. iii. Fluorescence method. iv. Tissue culture. v. Conductimetric assays. vi. Vegetative compatibility group analysis. 5. Seeding symptom test: 1. Blotter test. ii. Agar test. iii. Rolled paper towel test. iv. Soil test. v. Seedling paraquat test. vi. Bell method. vii. Plant growth medium. 6. Infectivity test: i. Fungi. ii. Bacteria. iii. Viruses. iv. Viroids. 7. Histopathological test: i. Whole seed stain. ii. Seed component. iii. Microtome seed sections. iv. Embryo count method. 8. Non-destructive seed assays: i. Biopsis assay. ii. Ultrasound analysis. III. Chlorophyll fluorescence. iv. High speed optical sorters. v. Near infrared reflectance spectroscopy (NIRS). vi. Fourier transform infrared (FTIR) photoacoustic. vii. Computer image analysis. 9. Bacteriophage tests. 10. Immunoassays: i. Precipitin test. ii. Enzyme linked immunosorbent assay (ELISA). iii. Dot-immunobinding assay (DIBA). iv. Single antibody dot immunoassay (SADI). v. Serologically specific electron microscopy (SSEM). vi. Immunofluorescence assay (IF). vii. Immunofluroscence colony staining (IFC). viii. Radiommunoassays (RISA). ix. Solid phase radioimmunoassay (SPRIA). x. Immunogold labelling. xi. Enzyme linked fluorescent assay (ELFA). xii.Disperse dyeimmunoassay (DIA). xiii. Solid phase immunosorbent methods. xiv. Direct immunostaining assay. xv. Vector based assays. 11. Nucleic acid based techniques: 1. Nucleic acid probes. ii. Restriction fragment length polymorphisms (RFLP) analysis. iii. Polymerase chain reaction (PCR). 12. Electrophoresis: i. Starch get electrophoresis. ii. Polyacrylamide gel electrophoresis. iii. Isozymen analysis. 13. Estimation of fungal metabolites: i. Chitin and glucosamines. ii. Ergosterol. iii. Volatile metabolites. 14. Estimation of fungicides on treated seeds: i. Visual observation of seeds for dye intensity. ii. Bioassay. iii. Colorimetric methods. Glossary. Acronyms and other abbreviations. References. Appendices. Index.
"This is the first comprehensive reference & textbook on seed healt.
The present book is a comprehensive account of all relevant information on seed health in the form of a text as well as reference book. The text of the present book is being divided into two parts. Part one mainly deals with the terminology, historical development of seed testing and seed health testing, objectives of seed health testing etc.
Part second provides a comprehensive account of information on the techniques used for the detection of seed born fungi, bacteria, viruses, viroids and nematod. The techniques hereby dealts include conventional methods such as examination of dry seeds, seed soak and washing test, culture tests, infectivity test, seedling symptom test, histopathology and molecular techniques such as immunoassays and nucleic acid base techniques. The procedures such as non-destructive seed assays, estimation of fungal metabolites, gel electrophoresis, size-selective sieving, computer image analysis not used on routine basis have also been discussed. In addition, a chapter on methods for the estimation of fungicides on treated seeds is also being discussed."
