Understandings in Cytogenetic Techniques

Contents: Foreword. Preface. 1. Historical perspectives of cytogenetics. 2. Chromosomes. 3. Chromosome preparation: pretreatment, fixation, processing, staining and mounting. 3. Cell cycle. 5. Microscopes in Biology. 6. Measurement of cells. 7. Mitotic chromosome preparation: (a) Bone marrow of mouse. (b) Fish chromosome (c) Amphibian chromosome (d) Onion root tip (e) Brain cells of Drosophila. 8. Preparation of special chromosomes: (a) Lamp brush chromosomes (b) Polytene chromosomes. 9. Meiotic chromosome preparation: (a) Meiosis in grasshopper (b) Meiosis in mouse. 10. Cell culture techniques: (a) Lymphocyte culture (b) Preparation of chromosome. 11. Karyotyping and chromosomal abnormalities. 12. Chromosome banding techniques: C banding, G banding, Q banding, R banding, N banding and T banding. 13. Barr body. 14. Pedigree analysis. 15. Model organism, Drosophila, Mutants, crossing experiments. Reference.